期刊
FUNGAL GENETICS AND BIOLOGY
卷 45, 期 10, 页码 1439-1447出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2008.08.001
关键词
Zygosaccharomyces rouxii; Na+/H+-antiporter; Salt tolerance; Potassium homeostasis; Alkali-metal-cation transporters
资金
- GA AS CR [IAA500110801]
- [MSMT LC531]
Antiporters exporting Na+ and K+ in exchange for protons are conserved among yeast species. The only exception so far has been Zygosaccharomyces rouxii, an osmotolerant species closely related to Saccharomyces cerevisiae. Z. rouxii was described as possessing one plasma-membrane antiporter transporting only Na+ (ZrSod2-22p in the CBS 732(T) type strain). We report the characterization of a second gene, ZrNHA1, encoding a new K+(Na+)/H+-antiporter capable of both K+ and Na+ export. Synteny analyses suggested that ZrSOD2-22 originated by single duplication of the ZrNHA1 gene. Substrate specificities and transport properties of ZrNha1p and ZrSod2-22p were compared upon heterologous expression in S. cerevisiae, and then directly in Z rouxii. Deletion mutants and phenotype analyses revealed that ZrSod2-22 antiporter is important for Na+ detoxification, probably together with ZrEna1 ATPase; ZrNha1p is indispensable to maintain potassium homeostasis and ZrEna1p is not, in contrast to the situation in S. cerevisiae, involved in this function. (c) 2008 Elsevier Inc. All rights reserved.
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