期刊
FUNGAL GENETICS AND BIOLOGY
卷 45, 期 6, 页码 897-911出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2007.12.001
关键词
aspergillus nidulans; Bem1; BemA; polar growth
资金
- Biotechnology and Biological Sciences Research Council [50/CFB17725] Funding Source: Medline
BemA, the orthologue of Saccharomyces cerevisiae Bem1p, was identified through genome sequence comparison. We have shown that it plays a similar role to Bem1p in yeast, acting as a cell growth protein. Deletion of the gene produced a moderately abnormal hyphal tip morphology, and had an extremely detrimental effect on conidiospore production, with development stalling after conidiophore vesicle production. It was also shown that BemA is required for vacuole fusion, similar to Bem1p. This role is dependent on the first SH3 domain of the protein, whose deletion has no detectable effect on cell growth. Localisation studies showed that BemA formed a clear cap at hyphal tips, analogous to the S. cerevisiae polarisome. The relationship between BemA and SepA, a spitzenkorper protein, was investigated. It was found that localisation of the proteins were interdependent, and a conditional double mutant was inviable. (c) 2007 Elsevier Inc. All rights reserved.
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