期刊
FUNGAL GENETICS AND BIOLOGY
卷 45, 期 3, 页码 253-265出版社
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2007.10.002
关键词
yeast; protoplasts regeneration; S. cerevisiae protoplasts; gene expression; microarrays; expression profiling; cell wall; stress and cell wall; wall biosynthesis
Global transcription profiling during regeneration of Saccharomyces cerevisiae protoplasts was explored. DNA microarrays measured the expression of 6388 genes and wall removal resulted initially in over-expression of 861 genes that decayed later on, a behaviour expected from a transient stress response. Kinetics of expression divided the genes into 25 clusters. Transcription of the genes from clusters 14-25 was initially up-regulated, suggesting that the grouped genes permitted cell adaptation to the removal of the wall. Clustering of genes involved in '' wall structure and biosynthesis '' showed that most of them had initially low levels of expression that increased along the process. Analysis by use of the T-profiler showed that the group of '' structural components of the wall '' was up-regulated after two hours and remained as such during the process. These results evoke the likeness and difference with the process occurring in Candida albicans. (c) 2007 Elsevier Inc. All rights reserved.
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