期刊
FUNGAL ECOLOGY
卷 5, 期 5, 页码 633-640出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funeco.2012.04.002
关键词
Below-ground detection; Ectomycorrhizal (ECM) fungi; Fungal conservation; Species persistence; Species-specific PCR; Stipitate hydnoid fungi
资金
- Scottish Natural Heritage, the Macaulay Development TrustScottish Forestry Commission-Forestry Research Scotland and Plantlife
- Scottish Executive (Rural and Environment Research and Analysis Directorate)
Fungi are a vital component of ecosystem biodiversity, but spend most of their lives hidden from view. Monitoring ectomycorrhizal (ECM) fungi has mostly relied on the abundance and distribution of above-ground sporocarps without consideration of their below-ground vegetative mycelium. Molecular methods may provide the means of obtaining this information and allow a more accurate determination of their possible decline and threat of extinction. Stipitate hydnoid sporocarp occurrence was recorded and mapped for 9 yr at two sites in Scotland, UK. Soil samples were collected at locations where sporocarps of Hydnellum aurantiacum, Hydnellum caeruleum, Phellodon niger or Sarcodon glaucopus had occurred 1-4 yr previously. Species-specific DNA was detected at all sporocarp locations and RNA was detected at 75 % of the locations indicating that these species remained below-ground and viable at the majority of locations for at least 4 yr in the absence of sporocarps. (C) 2012 Elsevier Ltd and The British Mycological Society. All rights reserved.
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