期刊
FUNGAL ECOLOGY
卷 4, 期 6, 页码 437-448出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funeco.2011.05.005
关键词
cDNA; Fungal community; Internal transcribed spacer; SSU; Trunk; Wood decomposition
资金
- Academy of Finland [121630, 128229]
- Academy of Finland (AKA) [128229, 121630, 128229, 121630] Funding Source: Academy of Finland (AKA)
Current knowledge of the succession of fungi in decaying wood is mostly based on fruit bodies and in vitro culture. Here, we investigated the changing community of metabolically active fungi during the decomposition of fallen Picea abies logs by directly extracting and barcode sequencing precursor rRNA. We also compared rRNA-derived amplicons of the 18S and ITS regions in 21 isolates and discuss the use of RNA as a marker of metabolically active fungi. The richness of active fungi, revealed as separated bands in DGGE, peaked in logs at an advanced stage of decay. Soft-rot fungi were common in the early stages but white- and brown-rot fungi became dominant as decay progressed. Ectomycorrhizal fungi were detected at an early stage, and they became the most abundant group in the late stages of succession. A comparison of rRNA-derived amplicons revealed that although ITS was detected in the form of precursor rRNA, introns within 18S rDNA were already spliced. As such, rRNA- and rDNA-derived amplicons would yield different profiles of active and total communities if profiling method is affected by amplicon length. (C) 2011 Elsevier Ltd and The British Mycological Society. All rights reserved.
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