期刊
FUNGAL ECOLOGY
卷 3, 期 4, 页码 284-287出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funeco.2010.05.002
关键词
Environmental sampling; ITS; Molecular ecology; Nuclear ribosomal genes; Sequence identification
资金
- Frontiers in Biodiversity Research Centre of Excellence (University of Tartu)
- Fungi in Boreal Forest Soils Network
We introduce an open source software utility to extract the highly variable ITS1 and ITS2 subregions from fungal nuclear ITS sequences, the region of choice for environmental sampling and molecular identification of fungi. Inclusion of parts of the neighbouring, very conserved, ribosomal genes in the sequence identification process regularly leads to distorted results. The utility is available for UNIX-type operating systems, including MacOS X, and processes about 1000 sequences per minute. (C) 2010 Elsevier Ltd and The British Mycological Society. All rights reserved.
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