期刊
FUNGAL BIOLOGY
卷 122, 期 11, 页码 1050-1063出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2018.08.008
关键词
Blumeria graminis f. sp hordei (Bgh); Blumeria graminis f. sp tritici (Bgt); Cross-kingdom RNA transfer; Double-stranded RNA; RNA interference; Small RNA
类别
资金
- Deutsche Forschungsgemeinschaft (DFG) [PA 861/14-1, SPP1819]
Small RNAs (sRNAs) play a key role in eukaryotic gene regulation, for example by gene silencing via RNA interference (RNAi). The biogenesis of sRNAs depends on proteins that are generally conserved in all eukaryotic lineages, yet some species that lack part or all the components of the mechanism exist. Here we explored the presence of the RNAi machinery and its expression as well as the occurrence of sRNA candidates and their putative endogenous as well as host targets in phytopathogenic powdery mildew fungi. We focused on the species Blumeria graminis, which occurs in various specialized forms (formae speciales) that each have a strictly limited host range. B. graminis f. sp. hordei and B. graminis f. sp. tritici, colonizing barley and wheat, respectively, have genomes that are characterized by extensive gene loss. Nonetheless, we find that the RNAi machinery appears to be largely complete and expressed during infection. sRNA sequencing data enabled the identification of putative sRNAs in both pathogens. While a considerable part of the sRNA candidates have predicted target sites in endogenous genes and transposable elements, a small proportion appears to have targets in planta, suggesting potential cross-kingdom RNA transfer between powdery mildew fungi and their respective plant hosts. (C) 2018 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据