期刊
FUNGAL BIOLOGY
卷 117, 期 1, 页码 62-70出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2012.11.006
关键词
Basidiomycete; Laccase; Lignin-modifying peroxidases; White-rot fungi; Wood degradation
类别
资金
- Academy of Finland [1133022, 138331]
- EC Marie Curie action [PERG08-GA-2010-276794]
- Academy of Finland (AKA) [138331, 138331] Funding Source: Academy of Finland (AKA)
Production of the oxidoreductive lignin-modifying enzymes - lignin and manganese peroxidases (MnPs), and laccase - of the white-rot basidiomycete Phlebia radiata was investigated in semi-solid cultures supplemented with milled grey alder or Norway spruce and charcoal. Concentrations of nutrient nitrogen and Cu-supplement varied also in the cultures. According to extracellular activities, production of both lignin peroxidase (LiP) and MnP was significantly promoted with wood as carbon source, with milled alder (MA) and low nitrogen (LN) resulting with the maximal LiP activities (550 nkat l(-1)) and noticeable levels of MnP (3 mu kat l(-1)). Activities of LiP and MnP were also elevated on high nitrogen (HN) complex medium when supplemented with spruce and charcoal. Maximal laccase activities (22 and 29 mu kat l(-1)) were obtained in extra high nitrogen (eHN) containing defined and complex media supplemented with 1.5 mM Cu2+. However, the nitrogen source, either peptone or ammonium nitrate and asparagine, caused no stimulation on laccase production without Cu-supplement. This is also the first report to demonstrate a new, on high Cu2+ amended medium produced extracellular laccase of P. radiata with pI value of 4.9, thereby complementing our previous findings on gene expression, and cloning of a second laccase of this fungus. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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