期刊
FUNGAL BIOLOGY
卷 116, 期 3, 页码 427-437出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2012.01.002
关键词
Fungal mating; MAT1-1; MAT1-2; Ophiostoma
类别
资金
- University of Pretoria
- National Research Foundation (NRF)
- DST/NRF Centre of Excellence in Tree Health Biotechnology (CTHB) South Africa
In heterothallic Ascomycota, two opposite but distinct mating types control all sexual processes. Using mating crosses, mating types were assigned to ten isolates of the heterothallic fungal species Ophiostoma quercus. Primers were subsequently designed to target the MAT1-1-1, MAT1-1-3 (of the mating type 1 idiomorph), and MAT1-2-1 (of the mating type 2 idiomorph) genes in these isolates. Results showed that all isolates contained the full gene sequence for the MAT1-2-1 gene. In addition, fragments of the MAT1-1-1 and MAT1-1-3 genes were sequenced from all isolates. These results were unexpected, as each isolate from a heterothallic species would typically contain only one of the two possible MAT idiomorphs. (C) 2012 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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