期刊
FUNGAL BIOLOGY
卷 114, 期 1, 页码 26-33出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.mycres.2009.09.008
关键词
Chytridiomycete; Fungus; PCR; Virus vector
类别
资金
- European Commission [QLRT-1999-1471]
Olpidium brassicae is a ubiquitous obligate root-infecting fungal pathogen. It is an important vector of a wide range of plant viruses. Olpidium isolates that infected brassica plants did not infect lettuce plants and vice-versa. Host range tests, PCR amplification and sequencing of the internal transcribed spacer (ITS) and 5.8S regions of 25 Olpidium isolates from brassica, carrot, cucumber and lettuce originating from four continents revealed differences between isolates. Based on their ability to infect lettuce and brassicas and the differences between their ITS1, 5.8S and ITS2 regions they could be separated into a number of distinct groups. Comparisons with other published sequences revealed two distinct genetic groups of brassica-infecting isolates, two distinct groups of lettuce-infecting isolates, one of which contained a carrot-infecting isolate and a distinct group comprising a cucumber-infecting isolate and a melon-infecting isolate. The possibility of the isolates belonging to three distinct species is discussed. (C) 2009 The British Mycological Society. Published by Elsevier Ltd. All rights reserved.
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