期刊
FUNCTIONAL & INTEGRATIVE GENOMICS
卷 11, 期 2, 页码 307-325出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-011-0213-8
关键词
Barley; Differentially expressed genes; Cold acclimation; Crown; Leaf; Metabolic pathways
资金
- Czech Republic National Agency for Agricultural Research [QH 81287]
- Czech Ministry of Agriculture [Mze0002700604]
- Ministry of Education, Youth and Sports [OC09032]
- Italian Ministry of Agriculture
We report a series of microarray-based comparisons of gene expression in the leaf and crown of the winter barley cultivar Luxor, following the exposure of young plants to various periods of low (above and below zero) temperatures. A transcriptomic analysis identified genes which were either expressed in both the leaf and crown, or specifically in one or the other. Among the former were genes responsible for calcium and abscisic acid signalling, polyamine synthesis, late embryogenesis abundant proteins and dehydrins. In the crown, the key organ for cereal overwintering, cold treatment induced transient changes in the transcription of nucleosome assembly genes, and especially H2A and HTA11, which have been implicated in cold sensing in Arabidopsis thaliana. In the leaf, various heat-shock proteins were induced. Differences in expression pattern between the crown and leaf were frequent for genes involved in certain pathways responsible for osmolyte production (sucrose and starch, raffinose, gamma-aminobutyric acid metabolism), sugar signalling (trehalose metabolism) and secondary metabolism (lignin synthesis). The action of proteins with antifreeze activity, which were markedly induced during hardening, was demonstrated by a depression in the ice nucleation temperature.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据