期刊
FUNCTIONAL & INTEGRATIVE GENOMICS
卷 10, 期 2, 页码 207-214出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s10142-009-0144-9
关键词
cDNA library; Disease-related genes; EST; Quantitative real-time PCR; Red-rot; Water-deficit stress-related genes; Sugarcane
资金
- Department of Biotechnology
- University Grants Commission, Government of India
Sugarcane is an important international commodity as a valuable agricultural crop especially in developing countries. Sequencing was carried out to generate > 35,000 expressed sequence tags (ESTs) from healthy as well as red-rot-infected tissue of Indian subtropical variety of sugarcane. Subsequent clustering with existing sugarcane ESTs in public databases identified 4,087 clusters, including 85 clusters that preferentially express upon Colletotrichum falcatum (red-rot) infection, which were previously unreported. Real-time reverse transcription-PCR profiling of selected EST clusters identified several sugarcane clusters that show differential expression in response to biotic and abiotic stress conditions. Twenty-five stress-related clusters showed > 2-fold relative expression during water-deficit stress in sugarcane. Similarly, EST clusters could be identified, which exhibit association with red-rot disease when assessed in red-rot-susceptible and red-rot-resistant varieties of sugarcane. Such EST clusters are good candidates for in-depth analysis to elucidate stress-responsive pathways in sugarcane and facilitate genetic manipulation to tailor this crop for tolerance to various stresses.
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