期刊
FRONTIERS OF ENVIRONMENTAL SCIENCE & ENGINEERING IN CHINA
卷 5, 期 4, 页码 526-532出版社
HIGHER EDUCATION PRESS
DOI: 10.1007/s11783-010-0261-7
关键词
microcystin-LR(MC-LR); biodegradation; Sphingopyxis sp; USTB-05; cell-free extract
资金
- National Natural Science Foundation of China [203777008]
- State Key Joint Laboratory of Environment Simulation and Pollution Control [09K08ESPCT]
- Educational Committee of Beijing
A promising bacterial strain for biodegrading microcystin-LR (MC-LR) as the sole carbon and nitrogen source was successfully isolated from Lake Dianchi, China. The strain was identified as Sphingopyxis sp. USTB-05, which was the first isolated MCs-biodegrading Sphingopyxis sp. in China. The average biodegradation rate of MC-LR by Sphingopyxis sp. USTB-05 was 28.8 mg center dot L-1 per day, which was apparently higher than those of other bacteria reported so far. The optimal temperature and pH for both strain USTB-05 growth and MC-LR biodegradation were 30A degrees C and 7.0, respectively. The release of MC-LR from the cyanobacterial cells collected from Lake Guishui and the biodegradation of MC-LR by both strain and cell-free extract (CE) were investigated. The results indicated that MC-LR with the initial concentration of 4.0 mg center dot L-1 in water was biodegraded by Sphingopyxis sp. USTB-05 within 4 d, while MC-LR with the initial concentration of 28.8 mg center dot L-1 could be completely removed in 3 h by CE of Sphingopyxis sp. USTB-05 containing 350 mg center dot L-1 protein. During enzymatic biodegradation of MC-LR, two intermediate metabolites and a dead-end product were observed on an HPLC chromatogram. Moreover, the similar scanning profiles of MC-LR and its metabolic products indicate that the Adda side-chain of MC-LR was kept intact in all products.
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