期刊
FREE RADICAL RESEARCH
卷 42, 期 8, 页码 725-736出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715760802337265
关键词
antioxidation; alpha(1)-microglobulin; red cell; ROS; heme
资金
- Swedish Research Council [2005-7144, 2005-6582]
- King Gustav V's 80-year Foundation
- Swedish Society for Medical Research, the Royal Physiographic Society in Lund
- Foundations of Greta and Johan Kock and Alfred Osterlund
- Swedish Foundation for International Cooperation in Research and Higher Education (STINT)
- Blood and Defence Network
- Lund University
- Crafoord Foundation
- Thelma Zoega Foundation
alpha(1)-Microglobulin is a 26 kDa plasma and tissue glycoprotein that belongs to the lipocalin protein superfamily. Recent reports show that it is a reductase and radical scavenger and that it binds heme and has heme-degrading properties. This study has investigated the protective effects of alpha(1)-microglobulin against oxidation by heme and reactive oxygen species in the human erythroid cell line, K562. The results show that alpha(1)-microglobulin prevents intracellular oxidation and up-regulation of heme oxygenase-1 induced by heme, hydrogen peroxide and Fenton reaction-generated hydroxyl radicals in the culture medium. It also reduces the cytosol of non-oxidized cells. Endogeneous expression of alpha(1)-microglobulin was up-regulated by these oxidants and silencing of the alpha(1)-microglobulin expression increased the cytosol oxidation. alpha(1)-microglobulin also inhibited cell death caused by heme and cleared cells from bound heme. Binding of heme to alpha(1)-microglobulin increased the radical reductase activity of the protein as compared to the apo-protein. Finally, alpha(1)-microglobulin was localized mainly at the cell surface both when administered exogeneously and in non-treated cells. The results suggest that alpha(1)-microglobulin is involved in the defence against oxidative cellular injury caused by haemoglobin and heme and that the protein may employ both heme-scavenging and one-electron reduction of radicals to achieve this.
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