期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 65, 期 -, 页码 1310-1314出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2013.09.017
关键词
Superoxide radical anion; Luminescence; NADPH oxidase; Redox cycling; L-012; Luminol; Redox probe; Free radicals
资金
- NIH [RO1 HL063119]
- JCET [POIG.01.01.02-00-069/09]
- European Union
L-012, a luminol-based chemiluminescent (CL) probe, is widely used in vitro and in vivo to detect NADPH oxidase (Nox)-derived superoxide (O-2(.-)) and identify Nox inhibitors. Yet understanding of the free radical chemistry of the L-012 probe is still lacking. We report that peroxidase and H2O2 induce superoxide dismutase (SOD)-sensitive, L-012-derived CL in the presence of oxygen. O-2(.-) alone does not react with L-012 to emit luminescence. Self-generated 02 during oxidation of L-012 and luminol analogs artifactually induce CL inhibitable by SOD. These aspects make assays based on luminol analogs less than ideal for specific detection and identification of O-2(.-) and NOX inhibitors. (C) 2013 Published by Elsevier Inc.
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