期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 59, 期 -, 页码 27-35出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2012.09.038
关键词
Lipid/protein malondialdehyde; Thiobarbituric acid; Lipid/protein hydroperoxides; Oxidative stress; Xylenol orange; Free radicals
资金
- Greek Ministry of Education, University of Patras, Greece
A simple and sensitive method is presented for the simultaneous quantification (spectrophotometric and spectrofluorimetric) of the main lipid and protein peroxidation products after their initial fractionation: free malondialdehyde (FrMDA), protein-bound malondialdehyde (PrMDA), total hydroperoxides (LOON), and protein hydroperoxides (PrOOH). FrMDA and PrMDA (released from proteins by alkaline hydrolysis) are measured after the reaction of MDA with thiobarbituric acid (TBA) under acidic conditions, by the specific fluorimetric quantification of the resulting MDA-(TBA)(2) adduct chromophore. The measurement of LOOH and PrOOH is based on the reaction of Fe3+ (resulting from the reaction of LOOH and PrOOH with Fe2+) with xylenol orange (XO) and the, photometric quantification of the resulting XO-Fe complex. The sensitivity of the assays for FrMDA/PrMDA and LOOH/PrOOH is 20 and 100 pmol respectively. The method was applied successfully on human plasma and can be used. for the evaluation of oxidative stress in both basic and clinical research. (c) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据