期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 52, 期 9, 页码 1700-1707出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2012.02.016
关键词
Senescence-accelerated mouse; Mass spectrometry; 8-Oxoguanosine; Oxidative damage; Free radicals
资金
- Key International Science and Technology Cooperation Projects of China [2006DFB31410]
- National Natural Science Foundation of China [81171028]
A sensitive and accurate isotope-diluted LC-MS/MS method was developed for determination of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxo-dGsn), derived from DNA, and 8-oxo-7,8-dihydroguanosine (8-oxo-Gsn), derived from RNA, in various tissue specimens obtained from normal SAMR1 and senescence-accelerated SAMP8 mice. An age-dependent accumulation of oxidative DNA and RNA damage was observed in all the organs examined, namely, the brain, liver, lungs, heart, kidneys, and testes. Among these, the brain samples exhibited the highest values for DNA damage. These age-related increases in the 8-oxoguanine content in DNA and RNA occurred more rapidly in SAMP8 than in SAMR1 mice. Age-related increases in the contents of 8-oxo-dGsn and 8-oxo-Gsn were also observed in the plasma and urine; however, the ratios of 8-oxo-Gsn to 8-oxo-dGsn in these samples were considerably higher (6 to 13) compared with the values for the samples derived from other tissues (roughly 1), indicating that measurement of 8-oxo-Gsn in urine could be a novel means of evaluating the aging process. (c) 2012 Elsevier Inc. All rights reserved.
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