期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 53, 期 6, 页码 1222-1225出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2012.06.036
关键词
Methionine sulfoxide; Anti-methionine sulfoxide antibody; Oxidative stress
资金
- Intramural Research Program of the National Heart, Lung, and Blood Institute
Methionine residues in protein can be oxidized by reactive oxygen or nitrogen species to generate methionine sulfoxide. This covalent modification has been implicated in processes ranging from normal cell signaling to neurodegenerative diseases. A general method for detecting methionine sulfoxide in proteins would be of great value in studying these processes, but development of a chemical or immunochemical technique has been elusive. Recently, an antiserum raised against an oxidized corn protein, DZS18, was reported to be specific for methionine sulfoxide in proteins (Arch. Biochem. Biophys. 485:35-40; 2009). However, data included in that report indicate that the antiserum is not specific. Utilizing well-characterized native and methionine-oxidized glutamine synthetase and aprotinin, we confirm that the antiserum does not possess specificity for methionine sulfoxide. (c) 2012 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据