期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 50, 期 11, 页码 1517-1525出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2011.02.027
关键词
NADPH oxidase; Reactive oxygen species; Redox-sensitive cell signaling; NF-kappa B activation; Lung inflammation; Leukocyte infiltration; Free radicals
资金
- National Center for Complementary and Alternative Medicine (NCCAM) [P01 AT002034]
In vitro and limited in vivo evidence suggests that reactive oxygen species derived from NADPH oxidases (NOX-ROS) play an important role in inflammatory responses by enhancing the activity of redox-sensitive cell signaling pathways and transcription factors. Here, we investigated the role of NOX-ROS in TNF alpha-induced acute inflammatory responses in vivo, using mice deficient in the gp91(phox) (NOX2) or p47(phox) subunits of NADPH oxidase. Age- and body weight-matched C57BL/6J wild-type (WT) and gp91(phox) or p47(phox) knockout mice were injected intraperitoneally with 50 mu g TNF alpha/kg bw or saline vehicle control and sacrificed at various time points up to 24 h. Compared to WT mice, gp91(phox-/-) mice exhibited significantly diminished (P<0.05) TNF alpha-induced acute inflammatory responses in the lungs but not other tissues, including heart, liver, and kidney, as evidenced by decreased activation of the redox-sensitive transcription factor NF-kappa B, and decreased gene expression of interleukin (IL)-1 beta, IL-6, TNF alpha, E-selectin, and other cellular adhesion molecules. Similar results were observed in p47(phox-/-) mice. Interestingly, decreased lung inflammation in knockout mice was accompanied by increased leukocyte infiltration into the lungs compared to other tissues. Our data suggest that phagocytic NOX-ROS signaling plays a critical role in promoting TNF alpha-induced, NF-kappa B-dependent acute inflammatory responses and tissue injury specifically in the lungs, which is effected by preferential leukocyte infiltration. (C) 2011 Elsevier Inc. All rights reserved.
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