期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 49, 期 2, 页码 237-244出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2010.04.008
关键词
alpha-tocopherol; Coenzyme Q(10); Replicative lifespan; Oxidative stress; Pro-oxidant; Lipid peroxidation; Saccharomyces cerevisiae
资金
- Australian Research
- Australian Postgraduate Award
Reactive oxygen species contribute to cellular ageing and an increased level of oxidative stress is often associated with ageing in many organisms. Supplementation of antioxidants has been advocated to decrease cellular oxidative stress and potentially extend lifespan. A genetically modified K6001 strain of Saccharomyces cerevisiae was employed to determine the effect of several antioxidants, including D-erythroascorbic acid, alpha-tocopherol and coenzyme Q(10) on yeast cell replicative ageing. The replicative lifespan of the K6001 strain was assessed by absorbance change as cells exhibited a linear growth in glucose medium. In this study, water-soluble D-erythroascorbic acid had little effect on cell replicative lifespan. However, supplementation of the growth medium with the lipophilic antioxidants alpha-tocopherol increased oxidative stress and decreased cell lifespan. The use of alpha-tocopherol analogues revealed that the antioxidant activity and the membrane retention ability of alpha-tocopherol were involved in the lifespan reduction effect. Supplementation with either coenzyme Q(10) alone, or in combination with alpha-tocopherol also led to a reduction in yeast replicative lifespan. This study highlights a potential pro-oxidant action of antioxidants. (C) 2010 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据