期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 48, 期 2, 页码 325-331出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2009.11.003
关键词
Nitric oxide; Nitrite; Mass spectrometry; Membrane inlet; Erythrocytes; Hemoglobin; Free radicals
资金
- NIH [GM25154]
- University of Florida
The reactions of nitrite with deoxygenated human erythrocytes were examined using membrane inlet mass spectrometry to detect the accumulation of NO in an extracellular solution. In this method an inlet utilizing a silicon rubber membrane is submerged in cell suspensions and allows NO to pass from the extracellular solution into the mass spectrometer. This provides a direct, continuous, and quantitative determination of nitric oxide concentrations over long periods without the necessity of purging the Suspension with inert gas. We have not observed accumulation of NO compared with controls on a physiologically relevant time scale and conclude that, within the limitations of the mass spectrometric method and our experimental conditions, erythrocytes do not generate a net efflux of NO after the addition of millimolar concentrations of nitrite. Moreover, there was no evidence at the mass spectrometer of the accumulation of a peak at mass 76 that would indicate N2O3, all intermediate that decays into NO and NO2. Inhibition of red cell membrane anion exchangers and aquaporins did not affect these processes. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据