期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 49, 期 10, 页码 1582-1593出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2010.08.022
关键词
Superoxide dismutase; Skeletal muscle; Denervation; Aging; Fiber-type grouping; Free radicals
资金
- IUSM-Northwest
In this study skeletal muscles from 1.5- and 10-month-old Cu/Zn superoxide dismutase (SOD1) homozygous knockout (JLSod1(-/-)) mice obtained from The Jackson Laboratory (C57B16/129SvEv background) were compared with muscles from age- and sex-matched heterozygous (JLSod1(+/-)) littermates. The results of this study were compared with previously published data on two different strains of Sod1(-/-) mice: one from Dr. Epstein's laboratory (ELSod1(-/-); C57B16 background) and the other from Cephalon, Inc. (CSod1(-/-); 129/CD-1 background). Grouping of succinate dehydrogenase-positive fibers characterized muscles of Sod1(-/-) mice from all three strains. The 10-month-old Sod1(-/-) C and JL mice displayed pronounced denervation of the gastrocnemius muscle, whereas the ELSod(-/-) mice displayed a small degree of denervation at this age, but developed accelerated age-related denervation later on. Denervation markers were up-regulated in skeletal muscle of 10-month-old JLSod1(-/-) mice. This study is the first to show that metallothionein mRNA and protein expression was up-regulated in the skeletal muscle of 10-month-old JLSod1(-/-) mice and was mostly localized to the small atrophic muscle fibers. In conclusion, all three strains of Sod1(-/-) mice develop accelerated age-related muscle denervation, but the genetic background has significant influence on the progress of denervation. (C) 2010 Elsevier Inc. All rights reserved.
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