期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 46, 期 2, 页码 119-126出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2008.09.034
关键词
Biotin-switch technique; S-nitrosylation; S-nitrosothiol; Redox; Cysteine; Thiol; Nitric oxide; Nitrosative stress; Free radicals
资金
- National Institutes of Health [P01-HL075443]
Protein S-nitrosylation, the posttranslational modification of cysteine thiols to form S-nitrosothiols, is a principle mechanism of nitric oxide-based signaling. Studies have demonstrated myriad roles for S-nitrosylation in organisms from bacteria to humans, and recent efforts have greatly advanced Our scientific understanding of how this redox-based modification is dynamically regulated during physiological and pathophysiological conditions, The focus of this review is the biotin-switch technique (BST), which has become a mainstay assay for detecting S-nitrosylated proteins in complex biological systems. Potential pitfalls and modern adaptations of the BST are discussed, as are future directions for this assay in the burgeoning field of protein S-nitrosylation. (C) 2008 Elsevier Inc. All rights reserved.
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