期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 47, 期 10, 页码 1401-1407出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2009.08.006
关键词
Boronic acids; Boronates; Peroxynitrite; Probes; Kinetics; Stopped flow; HPLC; Free radicals
资金
- NHLBI [HL063119]
In this study, we show that boronates, a class of synthetic organic compounds, react rapidly and stoichiometrically with peroxynitrite (ONOO-) to form stable hydroxy derivatives as major products. Using a stopped-flow kinetic technique, we measured the second-order rate constants for the reaction with ONOO-, hypochlorous acid (HOCl), and hydrogen peroxide (H2O2) and found that ONOO- reacts with 4-acetylphenylboronic acid nearly a million times (k = 1.6 x 10(6) M-1 s(-1)) faster than does H2O2 (k = 2.2 M-1 s(-1)) and over 200 times faster than does HOCl (k = 6.2 x 10(3) M-1 s(-1)). Nitric oxide and superoxide together, but not alone, oxidized boronates to the same phenolic products. Similar reaction profiles were obtained with other boronates. Results from this study may be helpful in developing a novel class of fluorescent probes for the detection and imaging of ONOO- formed in cellular and cell-free systems. (C) 2009 Elsevier Inc. All rights reserved.
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