期刊
FREE RADICAL BIOLOGY AND MEDICINE
卷 47, 期 4, 页码 389-400出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2009.05.002
关键词
Carbonate radical; Chondroitin sulfate; EPR; Extracellular matrix; Glycosaminoglycans; Hyaluronan; Hydroxyl radical; Nitrogen dioxide radical; Peroxynitrite; Free radicals
资金
- Australian Research Council
- National Health and Medical Research Council
- National Heart Foundation (Australia)
Glycosaminoglycans (long-chain polysaccharides) are major components of the extracellular matrix, glycocalyx, and synovial fluid. These materials provide strength and elasticity to tissues and play a key role in regulating cell behavior. Modifications to these materials have been linked to multiple human pathologies. Although modification may occur via both enzymatic and nonenzymatic mechanisms, there is considerable evidence for oxidant-mediated matrix damage. Peroxynitrite (ONOO-/ONOOH) is a potential mediator of such damage, as elevated levels of this oxidant are likely to be present at sites of inflammation. In this study we demonstrate that hyaluronan and chondroitin sulfate are extensively depolymerized by HO center dot and CO3 center dot-, but not NO2 center dot, which may be formed from peroxynitrite. Polymer fragmentation is shown to be dependent on the radical flux, to be O-2-independent, and to occur in a site-selective manner as indicated by the detection of disaccharide fragments. EPR spin trapping experiments with polymers, oligomers, and component monosaccharides, including C-13-labeled materials, have provided evidence for the formation of specific carbon-centered sugar-derived radicals. The time course of formation of these radicals is consistent with these species being involved in polymer fragmentation. (C) 2009 Elsevier Inc. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据