期刊
FORENSIC SCIENCE INTERNATIONAL-GENETICS
卷 7, 期 1, 页码 143-150出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigen.2012.09.001
关键词
Body fluid identification; mRNA marker; Microarray; qRT-PCR; DNA/RNA co-extraction
资金
- research project for practical use and advancement of forensic DNA analysis of Supreme Prosecutors' Office, Republic of Korea
In forensic science, identifying a tissue where a forensic specimen was originated is one of the principal challenges. Messenger RNA (mRNA) profile clearly reveals tissue-specific gene expression patterns that many attempts have been made to use RNA for forensic tissue identification. To systematically investigate the body-fluid-specific expression of mRNAs and find novel mRNA markers for forensic body fluid identification, we performed DNA microarray experiment with 24 Korean body fluid samples. Shannon entropy and Q-values were calculated for each gene, and 137 body-fluid-specific candidate genes were selected. By applying more stringent criteria, we further selected 28 candidate genes and validated them by RT-PCR and qRT-PCR. As a result, we suggest a novel combination of four body-fluid-specific mRNA makers: PPBP for blood, FDCSP for saliva, MSMB for semen and MSLN for vaginal secretion. Multiplex qRT-PCR assay was designed using the four mRNA markers and DNA/RNA co-extraction method was tested for forensic use. This study will provide a thorough examination of body-fluid-specifically expressed mRNAs, which will enlarge the possibility of practical use of RNA for forensic purpose. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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