期刊
FORENSIC SCIENCE INTERNATIONAL-GENETICS
卷 4, 期 2, 页码 68-72出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigen.2009.05.003
关键词
Canis lupus; Non-human genotyping; PCR multiplex; Sex identification
资金
- FCT [SFRH/BDE/15581/2006, SFRH/BD/19585/2004]
- Programa Operacional Ciencia e Inovacao [POCI 2010]
- VI Programa Quadro
- Fundação para a Ciência e a Tecnologia [SFRH/BDE/15581/2006, SFRH/BD/19585/2004] Funding Source: FCT
A previously described canine-specific 9-STR multiplex, now including two markers for sex determination, was tested for the genotyping of 23 wolves from Northern and Central Portugal. The samples were collected at necropsies and presented varying states of preservation. Complete profiles were obtained in 74% of the samples, partial profiles in 22% and one completely null pro. le. This survey revealed 15 alleles not previously described in dogs, distributed among 6 STR loci. It is shown that this genotyping system, previously tested in domestic dogs, can be reliably used for obtaining complete genetic profiles in wolves with a matching probability of 2.45 x 10(-9) and compatible sex identification, even in sub-optimal samples. Moreover, a population structure analysis using the observed genotypes revealed that this multiplexed 11-loci panel may potentially be used for discriminating between wolves and dogs. (C) 2009 Elsevier Ireland Ltd. All rights reserved.
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