期刊
FORENSIC SCIENCE INTERNATIONAL
卷 239, 期 -, 页码 11-18出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.forsciint.2014.02.029
关键词
Quantitative PCR; Ancient DNA; Bone density
资金
- National Institute of Justice, Office of Justice Programs, U.S. Department of Justice [2008-DN-BX-K008]
While recent forensic research has focused on determining which skeletal elements are superior in their preservation of DNA over the long term, little focus has been placed on measuring intra-element variation. Moreover, there is a general belief that dense (cortical) bone material will contain betterpreserved DNA than does spongy (cancellous) bone. To address these ideas, quantitative PCR was used to estimate the degree of mitochondrial DNA (mtDNA) preservation variance across sections of 19 northern fur seal ribs (Callorhinus ursinus) that date to similar to 3000 years before present. Further, we developed a measure called the density index'' that was used to gauge the relative densities of the rib sections studied here to determine if density was an appropriate predictor of preservation. The average preservation among the samples was significantly different (ANOVA, p = 1.9 X 10 (9)) with only 15% of the total variance observed within samples. However, 12 of the 19 specimens (similar to 63.2%) exhibited at least an order of magnitude difference in mtDNA preservation across the whole. Regression of the amount of mtDNA extracted per gram of bone material against the density index of the bone from which it was extracted demonstrates no relationship between these variables (R-2 = 0.03, p = 0.28). (C) 2014 Elsevier Ireland Ltd. All rights reserved.
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