期刊
FORENSIC SCIENCE INTERNATIONAL
卷 220, 期 1-3, 页码 251-264出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.forsciint.2012.03.012
关键词
Blowfly; Development; Forensic entomology; Metamorphosis; Micro-computed tomography; Post-mortem interval
资金
- Department for Business, Innovation and Skills (BIS, UK Government)
We demonstrate how micro-computed tomography (micro-CT) can be a powerful tool for describing internal and external morphological changes in Calliphora vicina (Diptera: Calliphoridae) during metamorphosis. Pupae were sampled during the 1st, 2nd, 3rd and 4th quarter of development after the onset of pupariation at 23 degrees C, and placed directly into 80% ethanol for preservation. In order to find the optimal contrast, four batches of pupae were treated differently: batch one was stained in 0.5 M aqueous iodine for 1 day; two for 7 days; three was tagged with a radiopaque dye; four was left unstained (control). Pupae stained for 7 d in iodine resulted in the best contrast micro-CT scans. The scans were of sufficiently high spatial resolution (17.2 mu m) to visualise the internal morphology of developing pharate adults at all four ages. A combination of external and internal morphological characters was shown to have the potential to estimate the age of blowfly pupae with a higher degree of accuracy and precision than using external morphological characters alone. Age specific developmental characters are described. The technique could be used as a measure to estimate a minimum post-mortem interval in cases of suspicious death where pupae are the oldest stages of insect evidence collected. (c) 2012 Elsevier Ireland Ltd. All rights reserved.
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