期刊
FORENSIC SCIENCE INTERNATIONAL
卷 207, 期 1-3, 页码 177-182出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.forsciint.2010.10.002
关键词
Alcohol; Biosensor; Multi-wall carbon nanotubes; Gold nanoparticles; Screen-printed electrodes
资金
- Scientific and Technological Project of Chongqing City, China [CSTC 2009AC5145]
A novel disposable amperometric biosensor strip for determination of blood alcohol concentration (BAC) with small volume of sample has been constructed using screen-printed electrodes (SPE), nanocomposite film and alcohol dehydrogenase (ADH). Firstly, the GNPs-MWCNT-Nafion nanocomposite film modified on a working electrode was made of Nafion-117, multi-wall carbon nanotubes (MWCNT) and gold nanoparticles (GNPs). After that Meldola's blue (MB) acted as an electron transfer mediator and the mixed solution of ADH, nicotinamide adenine dinucleotide (NAD+) were modified in order on the nanocomposite film. At last, a hydrophilic membrane which had an eyehole in center was placed at the outermost of the working area to make a reaction tank of 5 mu L, then the hydrophilic membrane/ADH-NAD(+)/GNPs-MWCNT-MB-Nafion/SPE was prepared. The detection of BAC can be accomplished with 5 mu L of blood sample obtained precisely by siphonage. Optimum conditions of the biosensor were experimentally determined by varying several important parameters: working potential, solution pH value, environmental temperature and interferences. Experimental results indicated that the biosensor possessed a good accuracy and stability, the linear response range was 2.0 x 10 (4) to 25 x 10 (3) mol/L and the detection limit of the biosensor was 5.0 x 10 (5) mol/L (S/N = 3). In the measurement of blood samples, the proposed biosensor had excellent detection performance for measuring BAC and showed a good correlation with gas chromatography. The prepared biosensor strip can be valid for the analysis of BAC. (C) 2010 Elsevier Ireland Ltd. All rights reserved.
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