Effects of Temperature and Nutrient Conditions on Biofilm Formation of Pseudomonas putida

In this study, biofilm formation of Pseudomonas panda at a constant temperature of 5 degrees C, 10 degrees C, 20 degrees C or 30 degrees C under rich and poor nutrient conditions was investigated. For all temperature conditions, R putida initially grew and formed biofilm. Subsequently, under the rich nutrient condition, the biofilm detached after it reached maturity at a high temperature, but those at a low temperature remained attached. In contrast, under the poor nutrient condition, biofilm detachment occurred regardless of the temperature condition; thus, lack of nutrients may cause biofilm detachment. Therefore, aside from cleaning blots and strict maintenance of a low temperature during the distribution of agricultural produce, attention must be paid to biofilm detachment as it can lead to an increased risk of bacterial contamination and food poisoning.