期刊
FOOD SCIENCE AND TECHNOLOGY RESEARCH
卷 15, 期 2, 页码 171-178出版社
JAPANESE SOC FOOD SCI & TECHNOLOGY
DOI: 10.3136/fstr.15.171
关键词
AOA; beta-carotene bleaching assay; DPPH assay; ORAC assay; Trolox; plate reader; tocopherol
资金
- Ministry of Agriculture, Forestry and Fisheries of Japan
The reproducibility and reliability of the micro-beta-carotene-linoleic acid bleaching (BCB) assay have been improved, enabling comparison to the antioxidant activity (AOA) of extracts from eleven kinds of crops evaluated by the oxygen radical absorbance capacity (ORAC) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays. All assays were conducted using 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (Trolox) as a calibrator, and AOAs were expressed as micromoles of Trolox equivalent per grain of weight. The coefficient variation (CV) of the BCB assay using a microplate was 14.40%, which was comparable to that of the ORAC assay (H-ORAC (14.10%), L-ORAC (18.76%)).
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