期刊
FOOD SCIENCE AND BIOTECHNOLOGY
卷 23, 期 4, 页码 1207-1211出版社
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-014-0165-6
关键词
phospholipid; phospholipase; lipase; structured lipid; organic solvent
The transesterification abilities of phospholipase A(2) and Mucor javanicus lipase in various organic solvents were studied. Phosphatidylcholine and caprylic acid (molar ratio, 1:6) were transesterified by incubation at 40A degrees C in three organic solvents using phospholipase A(2) and M. javanicus lipase. The fatty acid composition of the reaction product was measured using gas chromatography. Caprylic acid was incorporated into the sn-1 and -2 positions at a rate of 87.7% using phospholipase A(2) in hexane, and 36.7% using M. javanicus lipase in diethyl ether. Higher acyl migration into sn-2 was observed in diethyl ether than in hexane during transesterification using M. javanicus lipase, however, there was no substantial difference in the caprylic acid content at the sn-2 position. Acyl migration during transesterification in methanol was lower than in other organic solvents. Non-polar hexane and diethyl ether were more effective for transesterification than methanol.
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