期刊
FOOD SCIENCE AND BIOTECHNOLOGY
卷 21, 期 4, 页码 1135-1140出版社
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-012-0148-4
关键词
Lactobacillus brevis; Lactobacillus plantarum; mukeunji; probiotics
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2010-0011368]
- National Research Foundation of Korea [2010-0011368] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Lactic acid bacteria (LAB) isolated from mukeunji was screened for characterization of beneficial properties required as probiotics. Among the isolates from mukeunji, 5 LAB strains were selected due to their high levels of tolerance to simulated gastric fluid. These strains were identified by using SDS-PAGE of whole cell protein pattern and 16S rDNA sequencing. Subsequently, it was found that 2 isolates, Lactobacillus brevis C1 and Lactobacillus plantarum G4 among the 5 isolates were highly resistant to bile cytotoxicity in terms of survival rate, 162.2 and 114.3%, respectively. According to the results of in vitro adhesion assay, Lb. brevis C1, Lb. plantarum G4, and Lactobacillus sakei N1 had higher numbers of adhesion to Caco-2 epithelium cells than that of Lactobacillus rhamnosus GG, a well known probiotic. None of the isolates produced a carcinogenic enzyme, beta-glucuronidase. In addition, Lb. brevis C1 and Lb. plantarum G4 exhibited high levels of DPPH scavenging capacity. These results suggest that 2 isolates from mukeunji, Lb. brevis C1 and Lb. plantarum G4 may have potential for food products as probiotics because they showed high levels of bile acid tolerance and probiotic properties.
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