期刊
FOOD SCIENCE AND BIOTECHNOLOGY
卷 19, 期 6, 页码 1567-1571出版社
KOREAN SOCIETY FOOD SCIENCE & TECHNOLOGY-KOSFOST
DOI: 10.1007/s10068-010-0222-8
关键词
medium; optimization; exopolysaccharides (EPS) production; Cordyceps militaris; statistic experimental design
资金
- Hebei Education Department [2010128]
- China National Special Fund for State Key Laboratory of Biochemical Engineering [2010KF-12]
- Foundation of Tianjin Key Laboratory of Industrial Microbiology (Tianjin University of Science and Technology), P. R. China [GYWSW02]
Statistical experimental design strategy (SES) was applied to optimize the medium for the exopolysaccharides (EPS) production of Cordyceps militaris by submerged culture in shaker flask. A significant influence of the glucose and peptone on the EPS production was first evaluated by using a Plackett-Buman design. Then, steepest ascent method was employed to approach the experimental design space. Last, these factors were further optimized using central composite design (CCD) and response surface methodology (RSM). A quadratic model was found to fit the EPS production. The optimum values of the tested variables for the production of EPS were 48.67 g/L glucose, 12.56 g/L peptone, 1 g/L KH2PO4, 10 g/L yeast extract, and 0.5 g/L MgSO4 center dot 7H(2)O. Under optimization of culture conditions, the EPS production was enhanced from 0.78 to 1.96 g/L. In comparison with that of original culture conditions, 2.5 fold increase was obtained.
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