期刊
FOOD RESEARCH INTERNATIONAL
卷 54, 期 2, 页码 1699-1704出版社
ELSEVIER
DOI: 10.1016/j.foodres.2013.10.007
关键词
Lycium barbarum; ITS2 DNA barcode; Traceability
资金
- National Hightech R&D Program of China (863 Program) [2012AA021602]
- Fundamental Research Funds for the Central Universities [2012C02]
- Program for Changjiang Scholars and Innovative Research Team [IRT1150]
- Basic Scientific Research Operation Grants for State-Level Public Welfare Scientific Research Initiatives [YZ-12-08]
The recent horsemeat scandal and fish fraud have focused extensive attention on food safety worldwide. Plant-origin food safety must also be examined. Lycium barbarum, commonly known as Goji (or wolfberry), is considered a super food because of its nutritive properties, and it is much more expensive than L chinense and other varieties. However, distinguishing them merely by sight is difficult Thus, developing a useful tool for tracing food is crucial. In this study, we utilized an internal transcribed spacer 2 (ITS2) as a DNA barcode for distinguishing L. barbarism, L chinense, and L ruthencium. The genomic DNA from 67 fruit samples collected from the field and market was extracted. Ideal ITS2 barcodes were obtained by PCR amplification and sequencing. Based on the ITS2 barcode, the neighbor-joining (NJ) tree, BLAST1, and nearest distance methods were able to differentiate the three above-mentioned species. Therefore, we present the ITS2 barcode as a powerful tool for tracing Goji. (C) 2013 Elsevier Ltd. All rights reserved.
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