期刊
FOOD RESEARCH INTERNATIONAL
卷 41, 期 8, 页码 828-837出版社
ELSEVIER
DOI: 10.1016/j.foodres.2008.07.005
关键词
DNA barcoding; market survey; case study; mislabeled seafood; market substitution; species identification
资金
- Canadian Barcode of Life Network from Genome Canada (through the Ontario Genomics Institute)
- NSERC
Seafood authentication and food safety concerns are a growing issue in today's global marketplace, although traditional morphology-based identification keys and existing Molecular approaches have limitations for species identification. Recently, DNA barcoding has gained support as a rapid, cost-effective and broadly applicable molecular diagnostic technique for this purpose. However. the maturity of the barcode database as a tool for seafood authentication has yet to be tested using real market samples. The present case study was undertaken for this reason. Though the database is undergoing continual development, it was able to provide species matches of >97% sequence similarity for 90 of 91 samples tested. Twenty-five percent of the samples were potentially mislabeled, demonstrating that DNA barcodes are already a powerful tool for the identification of seafood to the species level. We conclude that barcodes have broad applicability for authenticity testing and the phylogeographic patterning of genetic diversity can also inform aspects of traceability. (C) 2008 Elsevier Ltd. All rights reserved.
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