期刊
FOOD MICROBIOLOGY
卷 44, 期 -, 页码 128-135出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2014.05.012
关键词
Mytilus galloprovincialis; Toxoplasma gondii Type I; Cyclospora cayetanensis; RealTime HRM; Public health
资金
- TUBITAK (Turkey) [CAYDAG 108Y185, CAYDAG 112Y020]
- PO Puglia FESR, Asse I, Linea 1.2. Accordo di Programma Quadro in materia di Ricerca Scientifica
To evaluate the presence of Toxoplasma gondii and Cyclospora cayetanensis in edible shellfish, a total of 795 specimens of Mytilus galloprovincialis from 8 different sites on the west coast of Turkey (Bays of Izmir and Mersin) were examined. Fifty-three pooled samples were created and subjected to EvaGreen (R) RealTime PCR assay and HRM analysis for protozoans detection and oocyst quantification. Seven out of 8 sites were contaminated with at least one protozoan species. Twenty-one out of 53 (39.6%) samples tested positive for T. gondii, C cayetanensis or both pathogens: 26.4% samples positive for C cayetanensis; 9.4% for T. gondii, and 3.8% for both protozoans. The number of oocysts in positive samples ranged from 6 to 30 for T gondii and from 3 to 48 for C cayetanensis. This is the first report of T. gondii Type 1, and C. cayetanensis in M. galloprovincialis, the most consumed shellfish species in Turkey. (C) 2014 Elsevier Ltd. All rights reserved.
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