期刊
FOOD MICROBIOLOGY
卷 34, 期 2, 页码 328-336出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2013.01.003
关键词
Clostridium perfringens; Food poisoning; Spore germination; Spore inactivation; Food contact surfaces; Disinfectants
资金
- Agricultural Research Foundation of Oregon State University
- Department of Defense Multidisciplinary University Research Initiative (MURI) through the U.S. Army Research Laboratory
- U.S. Army Research Office [W911NF-09-1-0286]
- Office of Higher Education Commission (Thailand)
- Ministry of Higher Education (Saudi Arabia)
The contamination of enterotoxigenic Clostridium perfringens spores on food contact surfaces posses a serious concern to food industry due to their high resistance to various preservation methods typically applied to control foodborne pathogens. In this study, we aimed to develop an strategy to inactivate C perfringens spores on stainless steel (SS) surfaces by inducing spore germination and killing of germinated spores with commonly used disinfectants. The mixture of L-Asparagine and KCl (AK) induced maximum spore germination for all tested C perfringens food poisoning (FP) and non-foodborne (NFB) isolates. Incubation temperature had a major impact on C perfringens spore germination, with 40 degrees C induced higher germination than room temperature (RT) (20 +/- 2 degrees C). In spore suspension, the implementation of AK-induced germination step prior to treatment with disinfectants significantly (p < 0.05) enhanced the inactivation of spores of FP strain SM101. However, under similar conditions, no significant spore inactivation was observed with NFB strain NB16. Interestingly, while the spores of FP isolates were able to germinate with AK upon their adhesion to SS chips, no significant germination was observed with spores of NFB isolates. Consequently, the incorporation of AK-induced germination step prior to decontamination of SS chips with disinfectants significantly (p < 0.05) inactivated the spores of FP isolates. Collectively, our current results showed that triggering spore germination considerably increased sporicidal activity of the commonly used disinfectants against C perfringens FP spores attached to SS chips. These findings should help in developing an effective strategy to inactivate C perfringens spores adhered to food contact surfaces. (C) 2013 Elsevier Ltd. All rights reserved.
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