期刊
FOOD MICROBIOLOGY
卷 34, 期 2, 页码 352-359出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2013.01.008
关键词
Cranberry; Probiotics; Anthocyanins; Phenolics; Proanthocyanidins
资金
- USDA National Needs Graduate Fellowship Competitive (award) [2007-38420-17764]
- UMaine Center for Excellence in Teaching and Assessment Active Student Learning Micro-Grants
- Maine Agricultural and Forest Experiment Station at the University of Maine [3306]
The antimicrobial properties of the American cranberry were studied against Escherichia coli O157:H7, Listeria monocytogenes, and Lactobacillus rhamnosus to determine the effects on growth inhibition, membrane permeability, and injury. Cranberry powder was separated using a C-18 Sep-Pak cartridge into sugars plus organic acids (F1), monomeric phenolics (F2), and anthocyanins plus proanthocyanidins (F3). Fraction 3 was further separated into anthocyanins (F4) and proanthocyanidins (F5) using an LH-20 Sephadex column. Each fraction was diluted in the brain heart infusion (BHI) broth to determine the minimum inhibitory/bactericidal concentrations (MIC/MBC). L monocytogenes was the most susceptible to cranberry fraction treatment with the lowest MIC/MBC for each treatment, followed by E. coli 0157:H7 and L rhamnosus. Membrane permeability and potential was studied using LIVE/DEAD viability assay and using Bis (1, 3-dibutylbarbituric acid) trimethine oxonol (DiBAC(4)), respectively. L. rhamnosus demonstrated the highest permeability followed by E. coli 0157:H7, and L monocytogenes. L rhamnosus demonstrated the highest recovery followed by E. coli 0157:H7, and L monocytogenes. Each cranberry fraction demonstrated membrane hyperpolarization at their native pH, while F2, F3, and F5 demonstrated membrane depolarization at neutral pH. With this knowledge cranberry compounds may be used to prevent maladies and potentially substitute for synthetic preservatives and antibiotics. (C) 2013 Elsevier Ltd. All rights reserved.
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