期刊
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
卷 52, 期 -, 页码 7-13出版社
ELSEVIER
DOI: 10.1016/j.jtice.2015.02.001
关键词
Hydrolysis; Lipolytic activity; Plackett-Burman design; Pseudomonas aeruginosa; Response surface methodology; Vegetable oils
资金
- National Natural Science Foundation of China [41273084]
- 863 Program [2013AA064403]
Lipase from the newly isolated Pseudomonas aeruginosa strain was produced, and its activity was enhanced through response surface methodology optimization. In the initial screening process, the bacterial strain was isolated from soil samples and then confirmed with lipase production on agar plates supplemented with Rhodamine B. The strain was identified by 16S rRNA gene sequence analysis. Plackett-Burman design was used to initially screen nine factors of culture medium. The design showed that four factors including peanut oil, sucrose, ammonium sulfate and volume of the medium exhibited significant affect on lipase production by P. aeruginosa strain. Afterward the optimal level of each significant factor was derived by Box-Behnken design and a 13.7 fold increase in lipase activity (21 U/mL-288 U/mL) was achieved under the optimum conditions. Thirty six percent of the soybean oil was converted into free fatty acids after hydrolyzing the oil for 12 h in the presence of optimized lipase. (C) 2015 Taiwan Institute of Chemical Engineers. Published by Elsevier B.V. All rights reserved.
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