期刊
FOOD MICROBIOLOGY
卷 28, 期 8, 页码 1514-1518出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2011.07.010
关键词
GTG(5) rep-PCR; Lactobacillus plantarum; Lactobacillus pentosus; Lactobacillus paraplantarum; Bacteriocin; Table olives
资金
- Oenological Biotechnology research group [2005BRDI/12/13]
- Spanish government [INIA-RM 2004-00015, AECI-A/6041/06]
Near one hundred isolates of Lactobacillus paraplantarum, Lactobacillus pentosus and Lactobacillus plantarum from table olives were studied. Strains were genotyped by rep-PCR. Although the technique failed to differentiate some isolates at the species level, it proved a robust and easy procedure that could be useful for distinguishing between related strains of L paraplantarum, L pentosus and L. plantarum from a large pool of unrelated strains of these species. A PCR-based screening revealed the presence of the plantaricin encoding genes plnA, plnB, plnC, plnD, plnE/F, plnF, plnI, plnJ plnK, plnG and plnN in most isolates of the three species. Sequences of bacteriocin genes present in L paraplantarum and L pentosus were homologous to L plantarum genes. Through a discriminating analysis of the bacteriocin gene profiles, it was possible to establish a relationship between the origin of isolation and the LAB isolates, regardless of species. (C) 2011 Elsevier Ltd. All rights reserved.
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