期刊
FOOD MICROBIOLOGY
卷 26, 期 5, 页码 542-546出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2009.01.008
关键词
Flow cytometry; Probiotic; Lactobacillus rhamnosus ATCC 53103; Thermal treatment; High pressure treatment; Inactivation pathway
资金
- Commission of the European Communities [QLKI-2000-30042]
The effect of thermal and pressure treatments on Lactobacillus rhamnosus ATCC 53103 was evaluated by flow cytometric analysis in conjunction to standard cultivation techniques. A double staining technique with fluorochromes carboxyfluorescein diacetate (cFDA) and propidium iodide (PI) revealed that depending on temperature regime used heat-killed cells had different fluorescence behaviors. Cells killed at 60 degrees C were not stained at all whereas heat treatment at 75 degrees C resulted in a single population entirely labelled by PI. These findings indicated that thermal-induced cell death was achievable with or without membrane degradation. Hydrostatic pressures beyond 400 MPa inactivated L. rhamnosus ATCC 53103 in a different way. It was observed that the irreversible damage of the membrane-bound transport systems could be largely accounted for the cause of high pressure-induced cell death. (c) 2009 Published by Elsevier Ltd.
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