期刊
FOOD MICROBIOLOGY
卷 26, 期 3, 页码 311-316出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fm.2008.12.006
关键词
Molecular detection methods; Foodborne pathogens; Salmonella; Listeria; Viable detection; Real-time PCR; Filtration
资金
- Agrarian Experimental Plan of the ITACyL/Junta de Castilla y Leon [2008/232211]
- European Union's Marie-Curie mobility program [MERG-CT-2007-209050]
We developed a novel filtration-based method that can eliminate dead or severally damaged Salmonella enterica and Listeria monocytogenes in food samples. This new method can recover all viable bacteria in less than 30 min, and can be coupled With a Subsequent bacterial DNA extraction and real-time PCR. No statically significant differences (p < 0.01) were found between real-time PCR results obtained separately from S. enterica and L. monocytogenes when different ratios of living and dead cells were used. The analytical sensitivity in both cases was I genome equivalent (GE), and the quantification was linear (R-2 > 0.9969) over a 5-log dynamic range with PCR efficiencies > 0.9754. When compared with the standard microbiological methods for the detection of these foodborne pathogens, the relative accuracy was excellent ranging from 95.72% to 104.48%. Finally, we applied the pre-treatment method to the direct detection of viable forms of these foodborne pathogens in food samples using yogurt as a model, the results being similar to those obtained using pure cultures. (C) 2009 Elsevier Ltd. All rights reserved.
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