Exopolysaccharides produced by Rhizobium radiobacter S10 in whey and their rheological properties

Exopolysaccharides (EPSs) produced by a number of bacteria genera are very attractive to food industries because of their stabilizing and emulsifying capabilities. Rhizobium radiobacter S10, an EPS-producing strain, was isolated from kefir. The chemical and rheological properties of the produced EPS were investigated. The EPS yield of the strain can reach 2834.2 mg L-1. The flow and viscoelastic behavior of S10 EPS were investigated by steady-shear and small-amplitude oscillatory experiments, respectively. The EPS solution (0.1%-1.0%, w/v) exhibited non-Newtonian and shear thinning behavior (pseudoplasticity) at shear rates that ranged from 0.01 s(-1) to 1000 s(-1). Weak gels were obtained with EPS concentrations as high as 0.75% at room temperature (25 degrees C). The changes in the elastic (G') and viscous (G '') moduli during the heating and cooling cycles indicated that S10 EPS can form a thermal reversible gel. The predominant fraction of S10 EPS, PK2, was composed of galactose and glucose at a molar ratio of 1.00:4.92 and had an approximate molecular weight of 3.03 x 10(6) Da. (C) 2013 Elsevier Ltd. All rights reserved.