期刊
FOOD HYDROCOLLOIDS
卷 36, 期 -, 页码 265-272出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2013.10.014
关键词
Leuconostoc citreum SK24.002; Water-soluble polysaccharide; Dextranase resistance; Structural characteristic
资金
- National Natural Science Foundation of China [31000764, 31230057]
- National High Technology Research and Development Program of China [2013AA102102]
- International Cooperative Program of of Jiangsu Province [BZ2012031]
- Science & Technology Pillar Program of Jiangsu Province [BE2012613, BY2012049, BE2013647]
Water-soluble polysaccharides that are produced from sucrose by lactobacillus strains isolated from Chinese traditional pickled vegetables were investigated. Strain SK24.002, which secreted an exopolysaccharide with a high dextranase resistance, was identified as Leuconostoc citreum via 16S rDNA sequence analysis. A polysaccharide yield of 35 g/L was achieved at 20 h in Man-Rogosa-Sharpe medium containing 100 g/L sucrose. The water-soluble polysaccharide consisted exclusively of glucose, and the weight-average molecular weight was 4.62 x 10(7) Da. Spectroscopy results demonstrated that the water-soluble exopolysaccharide was mainly composed of alpha-1,3 and alpha-1,6 linked D-glucopyranose units. Methylation analysis revealed that the ratio of alpha-1,6 linkages to alpha-1,3 linkages was 5:4. These results suggested a possible molecular structure for the water-soluble exopolysaccharide that consists of a backbone chain of alternating alpha-1,3 and alpha-1,6 linkages with a branched point at the C6 of the 1,3,6-linked D-glucopyranose unit. This novel polysaccharide may have applications as a functional ingredient in processed foods. (C) 2013 Published by Elsevier Ltd.
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