期刊
FOOD HYDROCOLLOIDS
卷 36, 期 -, 页码 339-347出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodhyd.2013.08.029
关键词
Ganoderma atrum; Polysaccharide; Molecular weight; Structure; NMR spectroscopy; Methylation
资金
- National Natural Science Foundation of China [21265011]
- National Key Technology R & D Program of China [2012BAD33B06]
- Key Program of National Natural Science Foundation of China [31130041]
- Program for New Century Excellent Talents in University [NCET-12-0749]
- Research Project of State Key Laboratory of Food Science and Technology [SKLF-ZZA-201301, SKLF-TS-201107]
- Special Fund of Jiangxi Province for the Innovation of Post-graduates [YC2012-B003]
A water-soluble heterogalactan, designated as PSG-2 was isolated and purified from the fruiting bodies of Ganoderma atrum. The molecular weight of PSG-2 was 6.9 x 10(4) Da by HPSEC determination. Its structure was analyzed using monosaccharide composition, partial acid hydrolysis, methylation analysis, combined with FT-IR spectra and 1D/2D NMR spectroscopy. PSG-2 was composed of galactose, fucose and glucose with molar percentage of 75.87%, 11.83% and 6.02%, respectively. Low content of mannose was also detected. Acetyl group was found in PSG-2 by FT-IR and NMR spectra. The backbone of PSG-2 consisted of 1,6-Galp (55.29%), and the short branched chains were attached to its O-2 position (1,2,6-Galp, 15.76%). The terminal residues contained T-Fucp (8.71%), T-Galp (5.97%) and T-Manp (1.83%). Three sugar residues, 1,2-Galp, 1,4-Glcp and 1,3-Glcp were also found to be with comparable amounts. Based on these results and NMR spectroscopy, a structure of PSG-2 was proposed as follows: [GRAPHICS] where R would be one of the following fragments: T-alpha-D-Galp(1 -> 6)-2-OAc-alpha-D-Galp-(1 ->, T-alpha-D-Galp(1 -> 6)-alpha-D-Galp-(1 -> 4)-beta-D-Glcp-(1 ->, T-alpha-D-Galp or T-alpha-D-Manp. (C) 2013 Elsevier Ltd. All rights reserved.
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