期刊
FOOD CONTROL
卷 96, 期 -, 页码 470-478出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2018.09.028
关键词
Bacteriocin CHQS; Antibacterial activity; Antibacterial action mode; Listeria ivanovii; Enterococcus faecalis
资金
- National Science-Technology Support Program of China [2015BAD16B01]
This study aims to examine the inhibitory effects of the bacteriocin CHQS, produced by Enterococcus faecalis TG2, and its mode of action against Listeria ivanovii DSMZ 20750(T), which is one of the pathogens sensitive to CHQS. The minimum inhibitory concentration, minimum bactericide concentration and time-inhibition curves were measured to confirm the strong effects of CHQS on L. ivanovii DSMZ 20750(T), and its antibacterial mode of action was studied by observing the changes in cell membrane permeability, integrity, and proton motive force. The minimum inhibitory concentration and minimum bactericide concentration of CHQS were 26.74 ng/mL and 107.30 ng/mL, respectively, demonstrating that CHQS exhibited significantly strong and rapid antibacterial efficacy towards L. ivanovii DSMZ 20750(T). The results showed that CHQS led to the leakage of K+ and electrolytes, the massive release of ATP and UV-absorbing materials, and the collapse of the proton motive force. The micrographs of scanning electron and transmission electron microscopy demonstrate the severe cellular destruction and release of intracellular substances induced by bacteriocin CHQS. Collectively, this study reveals the antibacterial activity and mode of action of CHQS against L. ivanovii DSMZ 20750(T) and demonstrates the mode of action of the inhibition by bacteriocins.
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