期刊
FOOD CONTROL
卷 42, 期 -, 页码 109-115出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2014.01.018
关键词
Tetracycline; Aptamer; Isothermal Titration Calorimetry; Electrochemical; Detection
资金
- Guangdong province Agricultural Science 82 Technology Program [2012A020200003]
- Scientific Cultivation and Innovation Fund Program of Jinan University [21612453]
- Key Laboratory of Bioengineering Medicine
- National Engineering Research Center
Developing the rapid, simple and sensitive biosensor system for tetracycline detection is very important in food safety. In this paper, we developed a label-free aptasensor for electrochemical detection of tetracycline. The reorganization of tetracycline binding aptamer was confirmed by Isothermal Titration Calorimetry, Kd = 5.18 Chi 10(-5) mol L-1. According to the electrochemical impendence spectroscopy (EIS) analysis, there was a linear relationship between the log concentration of tetracycline and the charge transfer resistance (Delta Ret) from 5.0 to 5.0 Chi 10(3) ng mL(-1) of the tetracycline conc. The detection limit was 1.0 ng mL(-1) within a detection time of 15 min. The average of assemble rate Q was at 82.4% with a differential batches' RSD of 4.6%. The current change of this aptasensor lies within at 8.5% after a storage of 15 days under 4 degrees C. The result aptasensor had shown a good reproducibility with an acceptable stability in tetracycline detection. The recoveries of TET in spiked milk samples were in the range of 90.0 95.7%. (C) 2014 Elsevier Ltd. All rights reserved.
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