期刊
FOOD CONTROL
卷 34, 期 1, 页码 121-125出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2013.04.023
关键词
Listeria spp.; Listeria monocytogenes; Raw milk; PCR serogrouping; Internalin genes; Antimicrobial resistance
资金
- PPP grant [PV123/2012A]
- High Impact Research Grant - Molecular Genetics from the University of Malaya [UM.C/625/1HIR/MOHE/ -02 [A000002-5000 1]]
A total of 446 various raw milk samples were collected from numerous farm bulk milk tanks to examine the presence of Listeria species. These isolates were further characterised by biochemical tests and Polymerase Chain Reaction (PCR). Listeria spp. was isolated in 83 of the 446 samples (18.6%). The highest prevalence of Listeria spp. was detected in raw cow milk samples (22.5%), followed by raw sheep milk (16.4%) and raw goat milk (4.9%). The most common species isolated was Listeria innocua (57.8%); the remaining Listeria isolates were Listeria monocytogenes (21.7%), Listeria welshimeri (12%), and Listeria seeligeri (8.4%). Based on PCR serotyping, the 18 L monocytogenes isolates were distributed into three serogroups 1/2a, 3a (n = 11), 1/2c, 3c (n = 5), and 4b, 4d, 4e (n = 2). All the examined L monocytogenes were positive for internalin genes (inlA, inlC, and inlJ). The Listeria spp. isolates were resistant to tetracycline (49.4%) and penicillin G (43.4%) but remained susceptible to gentamicin, vancomycin and rifampicin. The findings of this study show that consumption of raw milk could be a potential risk of human listeriosis. (C) 2013 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据